Hotspot

trnT-GGU-psbD

LSC · 33781-35222 · 1442 bp

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Pi 0.00562
MarkerSeek score 58.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1442
variable_sites
51
pi
0.00562
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTCCTTAACC 55.0 55.0 58.1 57.9 1575-1593 bp 1.0 82.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTCCTTAACC 50.0 55.0 58.1 57.9 1578-1596 bp 1.0 82.3
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1583-1601 bp 1.0 81.3
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1586-1604 bp 1.0 81.1
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG CGAAACGGTCCCTCCTTAAC 55.0 55.0 58.1 58.3 1576-1594 bp 1.0 81.1