Hotspot

petN-psbM

LSC · 30248-31308 · 1061 bp

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Pi 0.00619
MarkerSeek score 63.50
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1061
variable_sites
49
pi
0.00619
unique_haplotype_count
23
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCGTTGTTCATTCT 50.0 42.9 58.2 58.2 1222-1235 bp 1.0 72.3
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TGCTACTGCGTTGTTCATTC 50.0 45.0 58.2 56.7 1222-1235 bp 1.0 72.2
petN-psbM_p3 GGGACACGATTCACATGGAT TGCTACTGCGTTGTTCATTCT 50.0 42.9 57.7 58.2 1243-1256 bp 1.0 70.9
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT TGCTACTGCGTTGTTCATTCT 50.0 42.9 57.6 58.2 1161-1174 bp 1.0 70.9
petN-psbM_p5 GGGACACGATTCACATGGAT TGCTACTGCGTTGTTCATTC 50.0 45.0 57.7 56.7 1243-1256 bp 1.0 70.7