Hotspot

trnC-GCA-petN

LSC · 29093-30157 · 1065 bp

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Pi 0.00755
MarkerSeek score 61.20
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1065
variable_sites
52
pi
0.00755
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTTGTATCGTTTTGGCGGC AGCCCAAGCGAGACTTACTA 45.0 50.0 57.9 58.2 1158-1187 bp 1.0 84.2
trnC-GCA-petN_p2 CTGCAAATCCTTTCTCCCCA AGCCCAAGCGAGACTTACTA 50.0 50.0 57.8 58.2 1114-1143 bp 1.0 83.1
trnC-GCA-petN_p3 TGCAAATCCTTTCTCCCCAG AGCCCAAGCGAGACTTACTA 50.0 50.0 57.8 58.2 1113-1142 bp 1.0 83.1
trnC-GCA-petN_p4 ACTGCAAATCCTTTCTCCCC AGCCCAAGCGAGACTTACTA 50.0 50.0 57.8 58.2 1115-1144 bp 1.0 83.1
trnC-GCA-petN_p5 GCAAATCCTTTCTCCCCAGT AGCCCAAGCGAGACTTACTA 50.0 50.0 57.8 58.2 1112-1141 bp 1.0 83.1