Hotspot

rps16-trnQ-UUG

LSC · 6322-8196 · 1875 bp

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Pi 0.00868
MarkerSeek score 60.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1875
variable_sites
107
pi
0.00868
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1760-2014 bp 1.0 86.7
rps16-trnQ-UUG_p2 GGGTACAAGAATCCACAGCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.2 58.1 1793-2047 bp 1.0 85.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1764-2018 bp 1.0 85.6
rps16-trnQ-UUG_p4 GGGTACAAGAATCCACAGCC CTCGGAGGTTCGAATCCTTC 55.0 55.0 58.2 57.8 1797-2051 bp 1.0 84.7
rps16-trnQ-UUG_p5 GGTACAAGAATCCACAGCCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1792-2046 bp 0.962963 81.9