Hotspot

trnK-UUU-rps16

LSC · 4386-5206 · 821 bp

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Pi 0.00584
MarkerSeek score 64.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
821
variable_sites
41
pi
0.00584
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 830-974 bp 1.0 86.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 829-973 bp 1.0 86.3
trnK-UUU-rps16_p3 AAAAGACCATAGCCGCACTT AAAAAGGCGCTCAACCTACA 45.0 45.0 57.8 58.0 851-995 bp 1.0 86.0
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 839-983 bp 1.0 85.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 833-977 bp 1.0 84.7