Hotspot

trnH-GUG-psbA

LSC · 97-496 · 400 bp

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Pi 0.0353
MarkerSeek score 86.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
400
variable_sites
58
pi
0.0353
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.97
markerseek_score
86.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 410-478 bp 1.0 90.4
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 407-475 bp 0.962963 89.0
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 421-489 bp 0.962963 88.9
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 420-488 bp 0.962963 88.9
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 422-490 bp 0.962963 88.9