Hotspot

ycf1

SSC · 130816-136515 · 5700 bp

Back to Lithocarpus

Pi 0.00809

MarkerSeek score 53.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5700
variable_sites: 274
pi: 0.00809
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGAGAGAGACGCTTCAGGA	GGCGAACTAGGTTCAACCAT	50.0	50.0	58.1	57.9	867 bp	0.964286	81.0
ycf1_p2	AACCCTTACGGACCATCAGA	ATGGCGAACTAGGTTCAACC	50.0	50.0	58.1	57.9	1148 bp	0.892857	77.7
ycf1_p3	AACCCTTACGGACCATCAGA	GGCGAACTAGGTTCAACCAT	50.0	50.0	58.1	57.9	1146 bp	0.892857	77.7
ycf1_p4	ACCCTTACGGACCATCAGAA	ATGGCGAACTAGGTTCAACC	50.0	50.0	58.1	57.9	1147 bp	0.892857	77.7
ycf1_p5	ACCCTTACGGACCATCAGAA	GGCGAACTAGGTTCAACCAT	50.0	50.0	58.1	57.9	1145 bp	0.892857	77.7