Hotspot

ccsA-ndhD

SSC · 122097-122416 · 320 bp

Back to Lithocarpus

Pi 0.019

MarkerSeek score 69.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 320
variable_sites: 24
pi: 0.019
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AATTGTAGCCTCCCTGGGAT	TCAGTTGACAAGGTCGAAACT	50.0	42.9	58.1	57.5	465-492 bp	1.0	76.1
ccsA-ndhD_p2	ATTGTAGCCTCCCTGGGATT	TCAGTTGACAAGGTCGAAACT	50.0	42.9	58.1	57.5	464-491 bp	1.0	76.1
ccsA-ndhD_p3	TTCTGCAATTGTAGCCTCCC	TCAGTTGACAAGGTCGAAACT	50.0	42.9	57.9	57.5	471-498 bp	1.0	75.7
ccsA-ndhD_p4	TAGCCTCCCTGGGATTTCTT	TCAGTTGACAAGGTCGAAACT	50.0	42.9	57.7	57.5	460-487 bp	0.964286	72.8
ccsA-ndhD_p5	AGCCTCCCTGGGATTTCTTA	TCAGTTGACAAGGTCGAAACT	50.0	42.9	57.7	57.5	459-486 bp	0.964286	72.6