Hotspot

psaJ-rpl33

LSC · 73101-73585 · 485 bp

Back to Lithocarpus

Pi 0.00751

MarkerSeek score 65.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 29
pi: 0.00751
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGCTTATATTCC	TCGAACATCTTTCCCCTTGG	50.0	50.0	57.6	57.5	523-558 bp	1.0	81.5
psaJ-rpl33_p2	GGTTCGGGTTTTTAGCAGGT	TCGAACATCTTTCCCCTTGG	50.0	50.0	58.4	57.5	569-604 bp	1.0	80.8
psaJ-rpl33_p3	TGGTTCGGGTTTTTAGCAGG	TCGAACATCTTTCCCCTTGG	50.0	50.0	58.4	57.5	570-605 bp	1.0	80.8
psaJ-rpl33_p4	TCCGGATGCGCTTATATTCC	GCATCATTTCGAACAGCACA	50.0	45.0	57.6	57.4	570-605 bp	1.0	79.8
psaJ-rpl33_p5	GGTTCGGGTTTTTAGCAGGT	GCATCATTTCGAACAGCACA	50.0	45.0	58.4	57.4	616-651 bp	1.0	79.2