Hotspot

psbZ-trnG-UCC

LSC · 40745-41370 · 626 bp

Back to Lithocarpus

Pi 0.00698

MarkerSeek score 67.80

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 40
pi: 0.00698
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	701-802 bp	1.0	86.2
psbZ-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	702-803 bp	1.0	86.1
psbZ-trnG-UCC_p3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.3	58.1	699-800 bp	1.0	85.8
psbZ-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.3	58.1	700-801 bp	1.0	85.7
psbZ-trnG-UCC_p5	TTGCTTCTCCTGATGGTTGG	GAACCCGCATCTTCTCCTTG	50.0	55.0	57.8	58.6	697-798 bp	1.0	81.5