Hotspot

psbC-trnS-UGA

LSC · 39803-40062 · 260 bp

Back to Lithocarpus

Pi 0.0196

MarkerSeek score 70.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 260
variable_sites: 22
pi: 0.0196
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TTTATGGCATGCAGGAAGGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.2	58.1	428-452 bp	1.0	85.7
psbC-trnS-UGA_p2	TTTATGGCATGCAGGAAGGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.2	58.2	374-398 bp	1.0	85.0
psbC-trnS-UGA_p3	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	401-425 bp	1.0	84.8
psbC-trnS-UGA_p4	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	400-424 bp	1.0	84.2
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.4	58.2	347-371 bp	1.0	84.1