Hotspot

trnS-GCU-trnG-GCC

LSC · 9576-10564 · 989 bp

Back to Lithocarpus

Pi 0.00895
MarkerSeek score 74.20
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
989
variable_sites
48
pi
0.00895
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA CGAAGGACCCCTTAACCATT 50.0 50.0 58.0 57.2 902-1104 bp 1.0 81.1
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC CGAAGGACCCCTTAACCATT 50.0 50.0 58.0 57.2 901-1103 bp 1.0 81.1
trnS-GCU-trnG-GCC_p3 TAGTCCACTCAGCCATCTCC CGAAGGACCCCTTAACCATT 55.0 50.0 58.2 57.2 889-1091 bp 1.0 79.5
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA CGAAGGACCCCTTAACCATTT 50.0 47.6 58.0 57.9 902-1104 bp 1.0 78.9
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC CGAAGGACCCCTTAACCATTT 50.0 47.6 58.0 57.9 901-1103 bp 1.0 78.9