Hotspot

psbK-psbI

LSC · 8811-9207 · 397 bp

Back to Lithocarpus

Pi 0.0105

MarkerSeek score 66.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 397
variable_sites: 28
pi: 0.0105
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	CTTCACTCTTCACGTCCAGG	45.0	55.0	58.1	57.9	526-553 bp	0.928571	84.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	CTTCACTCTTCACGTCCAGG	50.0	55.0	57.9	57.9	521-548 bp	0.928571	84.4
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	TCACTCTTCACGTCCAGGAT	45.0	50.0	58.1	58.1	524-551 bp	0.928571	84.4
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	TCACTCTTCACGTCCAGGAT	50.0	50.0	57.9	58.1	519-546 bp	0.928571	84.4
psbK-psbI_p5	CTTTTGTTTGGCAAGCTGCT	TTCACTCTTCACGTCCAGGA	45.0	50.0	58.1	58.3	525-552 bp	0.928571	82.6