Hotspot

ycf1

SSC · 125863-131349 · 5487 bp

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Pi 0.00125

MarkerSeek score 53.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5487
variable_sites: 11
pi: 0.00125
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTACGTCTTTGAGCACGCAT	GGAAGATCCCGAAGTTCGTT	45.0	50.0	58.2	57.6	243-285 bp	1.0	77.1
ycf1_p2	TTATACCCCGGCGAAAATCC	GGAAGATCCCGAAGTTCGTT	50.0	50.0	57.7	57.6	212-254 bp	1.0	76.9
ycf1_p3	TTTACGTCTTTGAGCACGCA	GGAAGATCCCGAAGTTCGTT	45.0	50.0	58.4	57.6	244-286 bp	1.0	75.3
ycf1_p4	CCTTTGATTATACCCCGGCG	GGAAGATCCCGAAGTTCGTT	55.0	50.0	58.5	57.6	219-261 bp	1.0	75.1
ycf1_p5	CTTTGATTATACCCCGGCGA	GGAAGATCCCGAAGTTCGTT	50.0	50.0	57.5	57.6	218-260 bp	1.0	74.7