Hotspot

rbcL

LSC · 56332-57792 · 1461 bp

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Pi 0

MarkerSeek score 31.90

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1461
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	GTCAAAGGTTTCATTCACGCA	AAAAGGATTGGGCCGAGTTT	42.9	45.0	57.7	58.0	1619 bp	1.0	69.1
rbcL_p2	TGTCAAAGGTTTCATTCACGC	AAAAGGATTGGGCCGAGTTT	42.9	45.0	57.7	58.0	1620 bp	1.0	69.1
rbcL_p3	GTCAAAGGTTTCATTCACGCA	TGAATTATGCGCGCCTAGTT	42.9	45.0	57.7	58.1	1560 bp	1.0	68.8
rbcL_p4	GTCAAAGGTTTCATTCACGCA	TTACTGAATTATGCGCGCCT	42.9	45.0	57.7	58.1	1564 bp	1.0	68.8
rbcL_p5	TGTCAAAGGTTTCATTCACGC	TGAATTATGCGCGCCTAGTT	42.9	45.0	57.7	58.1	1561 bp	1.0	68.8