Hotspot

infA

LSC · 81224-81421 · 198 bp

Back to Liriope

Pi 0.00338

MarkerSeek score 48.00

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 198
variable_sites: 1
pi: 0.00338
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	AGCTTCGAGTTGTATCTCTCAC	GGGGTTACCTGGATGAAAGA	45.5	50.0	57.6	56.5	295-297 bp	1.0	50.0
infA_p2	GCTTCGAGTTGTATCTCTCACC	GGGGTTACCTGGATGAAAGA	50.0	50.0	58.6	56.5	294-296 bp	1.0	48.4
infA_p3	AGCTTCGAGTTGTATCTCTCAC	GGGGTTACCTGGATGAAAGAA	45.5	47.6	57.6	57.3	295-297 bp	1.0	48.0
infA_p4	TCGAGTTGTATCTCTCACCA	GGGGTTACCTGGATGAAAGA	45.0	50.0	55.3	56.5	291-293 bp	1.0	47.6
infA_p5	CTTCGAGTTGTATCTCTCACCA	GGGGTTACCTGGATGAAAGA	45.5	50.0	57.3	56.5	293-295 bp	1.0	47.3