Hotspot

atpF

LSC · 12295-13667 · 1373 bp

Back to Liriope

Pi 0.00255

MarkerSeek score 60.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1373
variable_sites: 6
pi: 0.00255
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCAGCTCGAA	AAAAAGGGCGAGTCAAGTGA	40.0	45.0	54.9	57.7	1547 bp	1.0	52.4
atpF_p2	ACTAATTTCGTCAGCTCGAA	GGGCGAGTCAAGTGATACAA	40.0	50.0	54.9	57.6	1542 bp	1.0	52.1
atpF_p3	ACTAATTTCGTCAGCTCGAA	AGGGCGAGTCAAGTGATACA	40.0	50.0	54.9	58.4	1543 bp	1.0	51.6
atpF_p4	ACTAATTTCGTCAGCTCGAA	AAAAGGGCGAGTCAAGTGAT	40.0	45.0	54.9	57.4	1546 bp	1.0	50.6
atpF_p5	ACTAATTTCGTCAGCTCGAA	AAGGGCGAGTCAAGTGATAC	40.0	50.0	54.9	57.3	1544 bp	1.0	49.7