Hotspot

psaC-ndhE

SSC · 119371-119784 · 414 bp

Back to Liriope

Pi 0

MarkerSeek score 59.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 414
variable_sites: 0
pi: 0
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	CGGAAGCAGCTATTGGTCTA	50.0	50.0	58.1	57.1	529-608 bp	1.0	75.9
psaC-ndhE_p2	CAATACATCTGTGGGGCAGG	CGGAAGCAGCTATTGGTCTA	55.0	50.0	58.3	57.1	505-584 bp	1.0	74.3
psaC-ndhE_p3	CCAATACATCTGTGGGGCAG	CGGAAGCAGCTATTGGTCTA	55.0	50.0	58.3	57.1	506-585 bp	1.0	74.3
psaC-ndhE_p4	CAGGCTCGTACACATTGAGT	CGGAAGCAGCTATTGGTCTA	50.0	50.0	57.6	57.1	489-568 bp	1.0	73.9
psaC-ndhE_p5	AGCTTTACATCCGTCCCAAG	CGGAAGCAGCTATTGGTCTA	50.0	50.0	57.6	57.1	535-614 bp	1.0	73.6