Hotspot

ndhF-rpl32

SSC · 114072-115026 · 955 bp

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Pi 0.00175
MarkerSeek score 56.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
955
variable_sites
3
pi
0.00175
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCTGTCGGAATAAGCA ACTGACTTTGCTAAAGAAAAAGCT 45.0 33.3 57.8 57.5 1125-1187 bp 1.0 47.1
ndhF-rpl32_p2 TGTTGCTGTCGGAATAAGCA ACTGACTTTGCTAAAGAAAAAGC 45.0 34.8 57.8 56.2 1125-1187 bp 1.0 44.4
ndhF-rpl32_p3 TGCTGTCGGAATAAGCAGAA ACTGACTTTGCTAAAGAAAAAGCT 45.0 33.3 57.2 57.5 1122-1184 bp 1.0 44.2
ndhF-rpl32_p4 TTGCTGTCGGAATAAGCAGA ACTGACTTTGCTAAAGAAAAAGCT 45.0 33.3 57.2 57.5 1123-1185 bp 1.0 44.2
ndhF-rpl32_p5 TGTTGCTGTCGGAATAAGCA CTGACTTTGCTAAAGAAAAAGCT 45.0 34.8 57.8 55.9 1124-1186 bp 1.0 44.2