Hotspot

trnE-UUC-trnT-GGU

LSC · 31767-32258 · 492 bp

Back to Liriope

Pi 0.00407

MarkerSeek score 44.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 492
variable_sites: 3
pi: 0.00407
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	593-599 bp	1.0	79.7
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	572-578 bp	1.0	79.2
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	569-575 bp	1.0	79.1
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	590-596 bp	1.0	78.5
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	595-601 bp	1.0	77.4