Hotspot

trnC-GCA-petN

LSC · 28456-29384 · 929 bp

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Pi 0

MarkerSeek score 49.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 929
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.5	58.0	1007-1011 bp	1.0	76.0
trnC-GCA-petN_p2	ATTTTGCACCGAATTTGGCG	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	58.6	58.0	1047-1051 bp	1.0	75.6
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	50.0	55.0	57.5	58.1	1059-1063 bp	1.0	75.1
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	50.0	55.0	57.5	58.1	1058-1062 bp	1.0	75.1
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	AGTAGTGCCCAACCCTAGAG	50.0	55.0	57.5	58.1	1077-1081 bp	1.0	75.0