Hotspot

trnS-GCU-trnG-UCC

LSC · 8437-9590 · 1154 bp

Back to Liriope

Pi 0.00173
MarkerSeek score 64.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1154
variable_sites
4
pi
0.00173
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 1083-1294 bp 1.0 73.9
trnS-GCU-trnG-UCC_p2 AAGTGATCAGAGGGAACGGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.1 58.2 1087-1298 bp 1.0 73.9
trnS-GCU-trnG-UCC_p3 ATCAGAGGGAACGGAGAGAG ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 1082-1293 bp 1.0 73.8
trnS-GCU-trnG-UCC_p4 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCAC 55.0 45.0 57.9 56.8 1083-1294 bp 1.0 73.8
trnS-GCU-trnG-UCC_p5 GATCAGAGGGAACGGAGAGA CGAACGAATCGCACTTTTACC 55.0 47.6 57.9 58.2 1086-1297 bp 1.0 73.8