Hotspot

trnK-UUU-rps16

LSC · 4038-4674 · 637 bp

Back to Liriope

Pi 0.00262
MarkerSeek score 60.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
637
variable_sites
3
pi
0.00262
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 749 bp 1.0 77.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 748 bp 1.0 77.6
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.5 58.2 735 bp 1.0 75.2
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG GGTGCTCAACCTACAGGAAC 50.0 55.0 58.6 58.2 760 bp 1.0 74.3
trnK-UUU-rps16_p5 TCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 45.0 55.0 57.3 58.2 758 bp 1.0 73.1