Hotspot

matK-trnK-UUU

LSC · 2919-4000 · 1082 bp

Back to Liriope

Pi 0.00154

MarkerSeek score 51.10

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1082
variable_sites: 3
pi: 0.00154
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTAATCGCAACGAGAATGGA	TCTGGGTTGCTAACTCAACG	42.9	50.0	57.5	57.8	1178-1179 bp	1.0	66.8
matK-trnK-UUU_p2	ACTAATCGCAACGAGAATGGA	AACGGTAGAGTACTCGGCTT	42.9	50.0	57.5	58.2	1162-1163 bp	1.0	66.8
matK-trnK-UUU_p3	ACTAATCGCAACGAGAATGGA	ACGGTAGAGTACTCGGCTTT	42.9	50.0	57.5	58.2	1161-1162 bp	1.0	66.8
matK-trnK-UUU_p4	TCGCAACGAGAATGGAATTTC	TCTGGGTTGCTAACTCAACG	42.9	50.0	57.3	57.8	1173-1174 bp	1.0	65.0
matK-trnK-UUU_p5	TCGCAACGAGAATGGAATTTC	ACGGTAGAGTACTCGGCTTT	42.9	50.0	57.3	58.2	1156-1157 bp	1.0	65.0