Hotspot

ycf1

SSC · 128697-134186 · 5490 bp

Back to Liriodendron

Pi 0.00595

MarkerSeek score 35.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5490
variable_sites: 49
pi: 0.00595
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGAATCCTCTGTCCAAGTG	AGGCACAACTTGAGCAATGA	50.0	45.0	56.8	58.0	229 bp	1.0	72.7
ycf1_p2	TGGAATCCTCTGTCCAAGTGA	AGGCACAACTTGAGCAATGA	47.6	45.0	58.4	58.0	229 bp	1.0	70.9
ycf1_p3	GGAATCCTCTGTCCAAGTGA	AGGCACAACTTGAGCAATGA	50.0	45.0	56.5	58.0	228 bp	1.0	70.4
ycf1_p4	TGGAATCCTCTGTCCAAGTG	GGCACAACTTGAGCAATGAG	50.0	50.0	56.8	57.7	228 bp	1.0	70.1
ycf1_p5	ATGGAATCCTCTGTCCAAGTG	AGGCACAACTTGAGCAATGA	47.6	45.0	57.4	58.0	230 bp	1.0	68.8