Hotspot

ccsA-ndhD

SSC · 120140-120366 · 227 bp

Back to Liriodendron

Pi 0.0235

MarkerSeek score 50.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 227
variable_sites: 8
pi: 0.0235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTGGCTTCCATGGGATTTC	TCCTGATTTCGTTCTCTCGC	50.0	50.0	58.1	57.7	403 bp	1.0	78.2
ccsA-ndhD_p2	GTGGCTTCCATGGGATTTCT	TCCTGATTTCGTTCTCTCGC	50.0	50.0	57.9	57.7	402 bp	1.0	78.2
ccsA-ndhD_p3	TGTGGCTTCCATGGGATTTC	CCTGATTTCGTTCTCTCGCT	50.0	50.0	58.1	57.7	402 bp	1.0	78.1
ccsA-ndhD_p4	GTGGCTTCCATGGGATTTCT	CCTGATTTCGTTCTCTCGCT	50.0	50.0	57.9	57.7	401 bp	1.0	78.1
ccsA-ndhD_p5	TTGTGGCTTCCATGGGATTT	TCCTGATTTCGTTCTCTCGC	45.0	50.0	57.7	57.7	404 bp	1.0	76.8