Hotspot

ndhF-rpl32

SSC · 116438-117573 · 1136 bp

Back to Liriodendron

Pi 0.00763

MarkerSeek score 51.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1136
variable_sites: 13
pi: 0.00763
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACAAGCAGAAGTCCCAA	TTTACCGCTGCCCAATATCC	50.0	50.0	58.0	58.0	1314 bp	1.0	80.4
ndhF-rpl32_p2	CAGTCGGAACAAGCAGAAGT	TTTACCGCTGCCCAATATCC	50.0	50.0	58.1	58.0	1319 bp	1.0	79.6
ndhF-rpl32_p3	AGTCGGAACAAGCAGAAGTC	TTTACCGCTGCCCAATATCC	50.0	50.0	57.8	58.0	1318 bp	1.0	79.3
ndhF-rpl32_p4	ACAAGCAGAAGTCCCAATCC	TTTACCGCTGCCCAATATCC	50.0	50.0	57.8	58.0	1311 bp	1.0	78.9
ndhF-rpl32_p5	CTGGAAGTGGAACGAAAGGT	TTTACCGCTGCCCAATATCC	50.0	50.0	57.7	58.0	1277 bp	1.0	78.6