Hotspot

trnI-CAU-ycf2

IRb · 89830-89897 · 68 bp

Back to Liriodendron

Pi 0.0196

MarkerSeek score 52.50

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 68
variable_sites: 2
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	TGTGAGTGAAAGATCCCACTG	50.0	47.6	58.0	57.6	212 bp	1.0	69.4
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	TCCCACTGAATCGAATTTGGT	50.0	42.9	58.0	57.6	199 bp	1.0	69.3
trnI-CAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	GTGAGTGAAAGATCCCACTGA	50.0	47.6	58.0	57.3	211 bp	1.0	67.2
trnI-CAU-ycf2_p4	GTTGGGCGCTTTAACCATTC	CCACTGAATCGAATTTGGTCC	50.0	47.6	58.0	57.2	197 bp	1.0	66.6
trnI-CAU-ycf2_p5	GTTGGGCGCTTTAACCATTC	CCCACTGAATCGAATTTGGTC	50.0	47.6	58.0	57.2	198 bp	1.0	66.6