Hotspot

petG-trnW-CCA

LSC · 69952-70065 · 114 bp

Back to Liriodendron

Pi 0.0351
MarkerSeek score 55.50
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
114
variable_sites
6
pi
0.0351
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 ACAATACAGACGCGGTGATC AGAACGTGGGTCTCCAAAAC 50.0 50.0 58.1 58.0 201-202 bp 1.0 81.5
petG-trnW-CCA_p2 CAATACAGACGCGGTGATCA AGAACGTGGGTCTCCAAAAC 50.0 50.0 58.1 58.0 200-201 bp 1.0 81.5
petG-trnW-CCA_p3 ACAATACAGACGCGGTGATC AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.1 58.0 223-224 bp 1.0 81.4
petG-trnW-CCA_p4 CAATACAGACGCGGTGATCA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.1 58.0 222-223 bp 1.0 81.4
petG-trnW-CCA_p5 ACAATACAGACGCGGTGATC CAAAACCCGATGTCGTAGGT 50.0 50.0 58.1 57.9 187-188 bp 1.0 81.2