Hotspot

petL-petG

LSC · 69661-69837 · 177 bp

Back to Liriodendron

Pi 0.00753

MarkerSeek score 31.50

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 177
variable_sites: 2
pi: 0.00753
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGAGCAAGATACGACTTATTTGA	GATCACCGCGTCTGTATTGT	34.8	50.0	55.8	58.1	300 bp	1.0	40.5
petL-petG_p2	TGAGCAAGATACGACTTATTTGA	TGATCACCGCGTCTGTATTG	34.8	50.0	55.8	58.1	301 bp	1.0	40.5
petL-petG_p3	TGAGCAAGATACGACTTATTTGA	AAAGGTCCAACTGATCACCG	34.8	50.0	55.8	57.8	312 bp	1.0	40.5
petL-petG_p4	TGAGCAAGATACGACTTATTTGA	CTGATCACCGCGTCTGTATT	34.8	50.0	55.8	57.8	302 bp	1.0	40.5
petL-petG_p5	TGAGCAAGATACGACTTATTTG	GATCACCGCGTCTGTATTGT	36.4	50.0	54.3	58.1	300 bp	1.0	40.5