Hotspot

rps14-psaB

LSC · 39897-40038 · 142 bp

Back to Liriodendron

Pi 0.0188
MarkerSeek score 52.80
Primer available yes
Label rps14-psaB

Feature Metadata

label_name
rps14-psaB
feature_type
igs
region
LSC
length_bp
142
variable_sites
4
pi
0.0188
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps14-psaB_p1 TGCCTTTTTCTCTCCCTCTG ACCAGTGGCTCTTTCCATTG 50.0 50.0 57.1 58.1 301-303 bp 1.0 73.7
rps14-psaB_p2 TGCCTTTTTCTCTCCCTCTG CCAGTGGCTCTTTCCATTGT 50.0 50.0 57.1 58.1 300-302 bp 1.0 73.7
rps14-psaB_p3 TGCCTTTTTCTCTCCCTCTG GCAGCTTTCTTGATTGCCTC 50.0 50.0 57.1 57.7 225-227 bp 1.0 72.6
rps14-psaB_p4 TGCCTTTTTCTCTCCCTCTG GCTCTTTCCATTGTGCAAGC 50.0 50.0 57.1 58.3 294-296 bp 1.0 72.1
rps14-psaB_p5 TGCCTTTTTCTCTCCCTCTG TGGCTCTTTCCATTGTGCAA 50.0 45.0 57.1 58.3 296-298 bp 1.0 72.0