Hotspot

trnfM-CAU-rps14

LSC · 39402-39593 · 192 bp

Back to Liriodendron

Pi 0.00412

MarkerSeek score 56.00

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 1
pi: 0.00412
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	GCTACCAAACTGCTCTACCC	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.0	58.0	306-336 bp	1.0	83.9
trnfM-CAU-rps14_p2	AAGGTTATGAGCCTTGCGAG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	325-355 bp	1.0	83.9
trnfM-CAU-rps14_p3	GATGTTGCGGAGACAGGATT	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	360-390 bp	1.0	83.9
trnfM-CAU-rps14_p4	TTTGAACCCGTGACCTCAAG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	342-372 bp	1.0	83.8
trnfM-CAU-rps14_p5	ACAGGATTTGAACCCGTGAC	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.1	58.0	348-378 bp	1.0	83.3