Hotspot

psbI-trnS-GCU

LSC · 9168-9309 · 142 bp

Back to Liriodendron

Pi 0.0141

MarkerSeek score 51.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 3
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCTGGACGCGAGGAATA	TGGACTAAAGCGACGGATTG	50.0	50.0	58.6	58.0	232-236 bp	1.0	77.6
psbI-trnS-GCU_p2	ATCCTGGACGCGAGGAATAA	TGGACTAAAGCGACGGATTG	50.0	50.0	58.6	58.0	231-235 bp	1.0	77.6
psbI-trnS-GCU_p3	TAATCCTGGACGCGAGGAAT	TGGACTAAAGCGACGGATTG	50.0	50.0	58.6	58.0	233-237 bp	1.0	77.6
psbI-trnS-GCU_p4	AATCCTGGACGCGAGGAATA	GTGGACTAAAGCGACGGATT	50.0	50.0	58.6	58.0	233-237 bp	1.0	77.6
psbI-trnS-GCU_p5	ATCCTGGACGCGAGGAATAA	GTGGACTAAAGCGACGGATT	50.0	50.0	58.6	58.0	232-236 bp	1.0	77.6