Hotspot

psaC

SSC · 122929-123174 · 246 bp

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Pi 0.007

MarkerSeek score 26.70

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 4
pi: 0.007
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	CTGTCAGCAAAATGGGTCCT	CTCATGAGCCGATCCAGAAG	50.0	55.0	58.1	57.8	788-790 bp	1.0	80.2
psaC_p2	TCCGCCAGTTGGATTAATGG	CTCATGAGCCGATCCAGAAG	50.0	55.0	57.9	57.8	705-707 bp	0.777778	71.3
psaC_p3	TCTTTCCTCCCCACATGGAT	CTCATGAGCCGATCCAGAAG	50.0	55.0	58.0	57.8	983-985 bp	0.777778	71.2
psaC_p4	TCTTTCCTCCCCACATGGAT	ATCTTGGCCCTCTCTCATGA	50.0	50.0	58.0	57.8	996-998 bp	0.777778	70.9
psaC_p5	TCTTTCCTCCCCACATGGAT	TCTCATGAGCCGATCCAGAA	50.0	50.0	58.0	58.2	984-986 bp	0.777778	70.6