Hotspot

ndhD-psaC

SSC · 122829-122928 · 100 bp

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Pi 0.0306

MarkerSeek score 55.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 100
variable_sites: 8
pi: 0.0306
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TAAGGAACCCGCGAATATCG	GCAGCATGGGTCTAGCTTAT	50.0	50.0	57.6	57.4	210-212 bp	1.0	75.5
ndhD-psaC_p2	TAAGGAACCCGCGAATATCG	GCAGCATGGGTCTAGCTTATT	50.0	47.6	57.6	58.1	210-212 bp	1.0	70.9
ndhD-psaC_p3	AGAAAATTAAGGAACCCGCGA	GCAGCATGGGTCTAGCTTAT	42.9	50.0	57.9	57.4	217-219 bp	1.0	70.3
ndhD-psaC_p4	CGCGAATATCGGCAAAACTAC	GCAGCATGGGTCTAGCTTAT	47.6	50.0	58.1	57.4	201-203 bp	1.0	70.2
ndhD-psaC_p5	CCGCGAATATCGGCAAAAC	GCAGCATGGGTCTAGCTTAT	52.6	50.0	57.9	57.4	202-204 bp	1.0	69.9