Hotspot

rpl32-tRNA-UAG

SSC · 118718-119961 · 1244 bp

Back to Liquidambar

Pi 0.00882

MarkerSeek score 48.30

Primer available yes

Label rpl32-tRNA-UAG

Feature Metadata

label_name: rpl32-tRNA-UAG
feature_type: igs
region: SSC
length_bp: 1244
variable_sites: 24
pi: 0.00882
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-tRNA-UAG_p1	TCTCTTTCTACCGGGAATTCA	AGCGTGTCTACCGATTTCAC	42.9	50.0	56.1	58.0	1330-1372 bp	1.0	59.4
rpl32-tRNA-UAG_p2	TCTCTTTCTACCGGGAATTCA	CAGCGTGTCTACCGATTTCA	42.9	50.0	56.1	58.0	1331-1373 bp	1.0	59.4
rpl32-tRNA-UAG_p3	TCTCTTTCTACCGGGAATTCA	TTTAGAAGATCCCATGCCGC	42.9	50.0	56.1	58.0	1395-1437 bp	1.0	59.0
rpl32-tRNA-UAG_p4	TCTCTTTCTACCGGGAATTCA	TCCTAAGAGCAGCGTGTCTA	42.9	50.0	56.1	57.9	1340-1382 bp	1.0	58.4
rpl32-tRNA-UAG_p5	TCTCTTTCTACCGGGAATTCA	CTAAGAGCAGCGTGTCTACC	42.9	55.0	56.1	57.8	1338-1380 bp	1.0	57.8