Hotspot

ndhF-rpl32

SSC · 117481-118552 · 1072 bp

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Pi 0.00705

MarkerSeek score 50.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1072
variable_sites: 19
pi: 0.00705
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAACGAAAGGT	TAACGCTGCCCTATATCCCT	50.0	50.0	57.7	57.7	1188-1211 bp	1.0	78.2
ndhF-rpl32_p2	CTGGAAGTGGAACGAAAGGT	GCTGCCCTATATCCCTTCCT	50.0	55.0	57.7	58.3	1184-1207 bp	1.0	78.1
ndhF-rpl32_p3	CTGGAAGTGGAACGAAAGGT	AACGCTGCCCTATATCCCTT	50.0	50.0	57.7	58.6	1187-1210 bp	1.0	76.3
ndhF-rpl32_p4	CGGAAAAAGGAGAAGTCCCA	TAACGCTGCCCTATATCCCT	50.0	50.0	57.4	57.7	1226-1249 bp	1.0	75.7
ndhF-rpl32_p5	CGGAAAAAGGAGAAGTCCCA	GCTGCCCTATATCCCTTCCT	50.0	55.0	57.4	58.3	1222-1245 bp	1.0	75.6