Hotspot

tRNA-UGG-psaJ

LSC · 71297-71706 · 410 bp

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Pi 0.0142

MarkerSeek score 38.50

Primer available yes

Label tRNA-UGG-psaJ

Feature Metadata

label_name: tRNA-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 15
pi: 0.0142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GACCTGCTAAAGACCCGAAC	50.0	55.0	58.1	58.3	538-543 bp	1.0	79.2
tRNA-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GACCTGCTAAAGACCCGAAC	50.0	55.0	58.1	58.3	537-542 bp	1.0	79.2
tRNA-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GACCTGCTAAAGACCCGAAC	50.0	55.0	58.1	58.3	549-554 bp	1.0	79.1
tRNA-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GACCTGCTAAAGACCCGAAC	50.0	55.0	58.1	58.3	548-553 bp	1.0	79.1
tRNA-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GACCTGCTAAAGACCCGAAC	50.0	55.0	58.1	58.3	551-556 bp	1.0	79.1