Hotspot

accD-psaI

LSC · 63044-63486 · 443 bp

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Pi 0.0133

MarkerSeek score 47.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 13
pi: 0.0133
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCGTTCTGAGTGAGTTA	ACTAAAGGCACCAAGACAGC	50.0	50.0	58.4	58.1	551-874 bp	1.0	78.7
accD-psaI_p2	AGGGCGTTCTGAGTGAGTTA	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.4	57.9	587-910 bp	1.0	78.5
accD-psaI_p3	AGGGCGTTCTGAGTGAGTTA	CAAGACAGCGGGTAAGTTGA	50.0	50.0	58.4	57.8	540-863 bp	1.0	78.2
accD-psaI_p4	AGGGCGTTCTGAGTGAGTTA	TTGCCATTGCCGGAAATACT	50.0	45.0	58.4	58.2	577-900 bp	1.0	78.1
accD-psaI_p5	AGGGCGTTCTGAGTGAGTTA	CCACTAAAGGCACCAAGACA	50.0	50.0	58.4	57.7	553-876 bp	1.0	77.3