Hotspot

psaB-psaA

LSC · 42703-42727 · 25 bp

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Pi 0.0156

MarkerSeek score 45.70

Primer available yes

Label psaB-psaA

Feature Metadata

label_name: psaB-psaA
feature_type: igs
region: LSC
length_bp: 25
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB-psaA_p1	TTCGAAGTCATGTGCGGTAG	ACAACATGGGCGTTCTTCTT	50.0	45.0	58.0	58.0	166 bp	1.0	80.3
psaB-psaA_p2	TTCGAAGTCATGTGCGGTAG	GTACAAGGACGTGCTGTAGG	50.0	55.0	58.0	58.0	217 bp	1.0	80.2
psaB-psaA_p3	TTCGAAGTCATGTGCGGTAG	GGGCGTTCTTCTTAGCAAGA	50.0	50.0	58.0	57.9	159 bp	1.0	79.7
psaB-psaA_p4	TTCGAAGTCATGTGCGGTAG	TACAAGGACGTGCTGTAGGA	50.0	50.0	58.0	58.1	216 bp	1.0	79.6
psaB-psaA_p5	GCTAAGCCTTGGCTAAACCT	GTACAAGGACGTGCTGTAGG	50.0	55.0	57.9	58.0	159 bp	1.0	79.4