Hotspot

psbC-tRNA-UGA

LSC · 38420-38658 · 239 bp

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Pi 0.0146
MarkerSeek score 45.00
Primer available yes
Label psbC-tRNA-UGA

Feature Metadata

label_name
psbC-tRNA-UGA
feature_type
igs
region
LSC
length_bp
239
variable_sites
9
pi
0.0146
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-tRNA-UGA_p1 CCTGTTCTTTCCATGACCCC GGGTTCGAATCCCTCTCTCT 55.0 55.0 58.2 58.2 270-296 bp 1.0 83.5
psbC-tRNA-UGA_p2 TCGTAGGTCATTTGTGGCAC GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.2 58.2 350-376 bp 1.0 82.7
psbC-tRNA-UGA_p3 AGCTGCAGCAGGATTTGAAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 58.4 58.2 313-339 bp 1.0 81.5
psbC-tRNA-UGA_p4 GCTGCAGCAGGATTTGAAAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.6 58.2 312-338 bp 1.0 80.9
psbC-tRNA-UGA_p5 TTCGTAGGTCATTTGTGGCA GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.4 58.2 351-377 bp 1.0 79.8