Hotspot

petN-psbM

LSC · 30494-31484 · 991 bp

Back to Liquidambar

Pi 0.00847
MarkerSeek score 57.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
991
variable_sites
22
pi
0.00847
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1159-1174 bp 1.0 69.1
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1098-1113 bp 1.0 67.6
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTC 50.0 45.0 58.2 56.1 1159-1174 bp 1.0 64.9
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTC 50.0 45.0 57.6 56.1 1098-1113 bp 1.0 63.4
petN-psbM_p5 ATAGTAAGTCTCGCTTGGGC TGCTACTGCACTGTTCATTCT 50.0 42.9 57.1 57.6 1160-1175 bp 1.0 63.2