Hotspot

atpH-atpI

LSC · 14972-16135 · 1164 bp

Back to Liquidambar

Pi 0.00538

MarkerSeek score 46.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1164
variable_sites: 14
pi: 0.00538
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCGGCAGAAATCAGTGGATT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.9	58.5	1212-1243 bp	1.0	72.0
atpH-atpI_p2	GCGGCAGAAATCAGTGGATT	AGGCGAATCTATGGAGGGTC	50.0	55.0	58.9	58.7	1184-1215 bp	1.0	71.0
atpH-atpI_p3	GCGGCAGAAATCAGTGGATT	TAGGCGAATCTATGGAGGGT	50.0	50.0	58.9	57.3	1185-1216 bp	1.0	70.7
atpH-atpI_p4	CGGCAGAAATCAGTGGATTC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	56.9	58.5	1211-1242 bp	1.0	70.3
atpH-atpI_p5	GGAAGCGGCAGAAATCAGT	TTTTTGCAACTTTAGCCGCG	52.6	45.0	58.1	58.5	1216-1247 bp	1.0	70.1