Hotspot

atpF-atpH

LSC · 14258-14725 · 468 bp

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Pi 0.0124

MarkerSeek score 41.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 13
pi: 0.0124
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TACGCCAACTCTTGTGGATG	50.0	50.0	58.0	57.9	1236-1256 bp	1.0	81.8
atpF-atpH_p2	AGAAAGAGTACCATGCTGCG	TACGCCAACTCTTGTGGATG	50.0	50.0	58.0	57.9	1588-1608 bp	1.0	81.6
atpF-atpH_p3	AGAAAGAGTACCATGCTGCG	AAGTCCTTCCGTTTCGTCTG	50.0	50.0	58.0	57.8	1408-1428 bp	1.0	81.3
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	AAGGTATTGCGAGACAACCC	50.0	50.0	58.0	57.9	627-647 bp	0.777778	74.0
atpF-atpH_p5	AGAAAGAGTACCATGCTGCG	AAGGTATTGCGAGACAACCC	50.0	50.0	58.0	57.9	979-999 bp	0.777778	73.0