Hotspot

ycf1

SSC · 123128-128527 · 5400 bp

Back to Liparis

Pi 0.0449

MarkerSeek score 76.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5400
variable_sites: 933
pi: 0.0449
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTGGGACAGGGAAGATA	CCTTTGGTTCTCCCCGAAAA	50.0	50.0	58.0	58.0	734-836 bp	0.789474	83.2
ycf1_p2	TATGCTTGGGACAGGGAAGA	CCTTTGGTTCTCCCCGAAAA	50.0	50.0	58.0	58.0	826-838 bp	0.578947	70.8
ycf1_p3	TGCTTGGGACAGGGAAGATA	CCCGAGTTGAACGAGGATTT	50.0	50.0	58.0	57.9	1001-1086 bp	0.315789	62.9
ycf1_p4	TGCTTGGGACAGGGAAGATA	AATTCCCGAGTTGAACGAGG	50.0	50.0	58.0	57.9	1005-1090 bp	0.315789	62.8
ycf1_p5	TATGCTTGGGACAGGGAAGA	CCCGAGTTGAACGAGGATTT	50.0	50.0	58.0	57.9	1085 bp	0.105263	43.9