Hotspot

rps16

LSC · 4776-5864 · 1089 bp

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Pi 0.0431

MarkerSeek score 81.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1089
variable_sites: 168
pi: 0.0431
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GTTGAGTTAGCAACCCGGAT	GACCAAATCAAAATGGGGCG	50.0	50.0	57.9	58.0	1996-2208 bp	0.947368	88.9
rps16_p2	AAAGCCGAGTACTCTACCGT	GACCAAATCAAAATGGGGCG	50.0	50.0	58.2	58.0	2014-2226 bp	0.947368	88.5
rps16_p3	AAGCCGAGTACTCTACCGTT	GACCAAATCAAAATGGGGCG	50.0	50.0	58.2	58.0	2013-2225 bp	0.947368	88.5
rps16_p4	GTTGAGTTAGCAACCCGGAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1935-2147 bp	0.947368	88.4
rps16_p5	GTTGAGTTAGCAACCCGGAT	AAATCCCAGCGACAACATGT	50.0	45.0	57.9	58.1	2108-2271 bp	0.421053	67.2