Hotspot

ndhF

SSC · 109717-111963 · 2247 bp

Back to Liparis

Pi 0.0393

MarkerSeek score 73.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 338
pi: 0.0393
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GTTCCGCGTTAGCCAGATTA	AGAATTGGTGAATCGGAAACA	50.0	38.1	58.1	55.8	2481-2972 bp	0.473684	44.5
ndhF_p2	CGCGTTAGCCAGATTAGTCA	AGAATTGGTGAATCGGAAACA	50.0	38.1	57.8	55.8	2477-2968 bp	0.473684	43.5
ndhF_p3	ACAGGAACAAGAGGGATCCA	AGAATTGGTGAATCGGAAACA	50.0	38.1	58.0	55.8	2108-2599 bp	0.263158	35.9
ndhF_p4	AAGAGGGATCCACCGAAGAA	AGAATTGGTGAATCGGAAACA	50.0	38.1	58.0	55.8	2100-2591 bp	0.263158	35.9
ndhF_p5	AGAGGGATCCACCGAAGAAA	AGAATTGGTGAATCGGAAACA	50.0	38.1	58.0	55.8	2099-2590 bp	0.263158	35.9