Hotspot

psaC-ndhE

SSC · 116796-117377 · 582 bp

Back to Liparis

Pi 0.0782

MarkerSeek score 79.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 582
variable_sites: 149
pi: 0.0782
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGACAAGCGGATTCACATCT	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.0	58.0	757-1007 bp	0.947368	89.5
psaC-ndhE_p2	AATACATCCGTGGGACAAGC	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.0	58.0	658-908 bp	0.894737	87.4
psaC-ndhE_p3	GGACAAGCGGATTCACATCT	AAGCCGAAGCATGGTTAGAG	50.0	50.0	58.0	58.0	937-1011 bp	0.736842	81.0
psaC-ndhE_p4	AATACATCCGTGGGACAAGC	GAGATAGGATCGCTTCGCTG	50.0	55.0	58.0	58.0	1355-1398 bp	0.368421	63.2
psaC-ndhE_p5	GGACAAGCGGATTCACATCT	GAGATAGGATCGCTTCGCTG	50.0	55.0	58.0	58.0	1454-1497 bp	0.368421	63.0