Hotspot

rpl32-trnL-UAG

SSC · 112926-113509 · 584 bp

Back to Liparis

Pi 0.061

MarkerSeek score 82.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 584
variable_sites: 139
pi: 0.061
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	1228-1354 bp	0.947368	89.2
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	1227-1353 bp	0.947368	89.2
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	961-1087 bp	0.947368	88.9
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	636-715 bp	0.947368	88.6
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	1108-1234 bp	0.894737	86.9