Hotspot

clpP-psbB

LSC · 71324-72064 · 741 bp

Back to Liparis

Pi 0.0663

MarkerSeek score 82.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 177
pi: 0.0663
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGGATCAAGAACAGGGTCAG	50.0	55.0	58.0	58.0	916-1065 bp	1.0	91.9
clpP-psbB_p2	CGCACTATACGTCAACCCAA	TAGACCCGCTAATATGCCCA	50.0	50.0	58.0	58.0	1396-1545 bp	1.0	91.8
clpP-psbB_p3	CGCACTATACGTCAACCCAA	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1259-1408 bp	0.947368	89.7
clpP-psbB_p4	TATACGTCAACCCAAGCTGC	CGGATCAAGAACAGGGTCAG	50.0	55.0	58.0	58.0	936-990 bp	0.631579	77.0
clpP-psbB_p5	CGCACTATACGTCAACCCAA	TGCACCAAAGCCAAAACAAG	50.0	45.0	58.0	58.0	1245-1298 bp	0.421053	68.7